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. 2019 Mar 27;9:5281. doi: 10.1038/s41598-019-41717-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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MiR-125a-5p inhibits FOXS1 expression by translational repression. (A) Three independent miRNA target databases were used to predict the potential miRNAs. (B) Schematic representation of the potential miRNAs binding sites in 3′UTR of FOXS1. (C) RT-PCR results of the expression of FOXS1 in the miRNA mimics of miR-328-3p or miR-125a-5p transfection group in BGC823 and HGC-27cells. (D) Levels of FOXS1 protein were analyzed by WB after transfection with mimics of miR-328-3p or miR-125a-5pfor 72 hours. (E) RT-PCR results of the expression of FOXS1 in GES-1 cells transfected with miRNA inhibitors of miR-125a-5p or nc. (F) Levels of FOXS1 protein were analyzed by WB after transfection with inhibitors of miR-125a-5p for 72 hours. (G) Predicted binding sites and mutant binding site between miR-125a-5p and 3′UTR OF FOXS1. (H) HGC-27 cells were firstly transfected with miR-125a-5p inhibitors or inhibitors nc and next day transfected with a luciferase reporter-containing FOXS1 wild-type 3′UTR (3′UTR-WT) or FOXS1 mutant 3′UTR (3′UTR-MUT). Luciferase activity of the indicated cells were determined by Luciferase assay kits.