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. 2019 Mar 27;9:5263. doi: 10.1038/s41598-019-41704-1

Table 5.

Sequence of the primers used for molecular identification of Aspergillus section Flavi isolates.

Gene Gene name Length bp Primers Sequence (Nucleotides: 5′→3′)
Forward Reverse
ITS (1–2) 18S ribosomal RNA gene partial sequence Internal Transcribed Spacer 565–613 ITS1 5′-GGAAGTAAAGTCGTAACAAGG
ITS 2 5′-TTGGTCCGTGTTTCAAGACG
ITS (4–5) 300–330 ITS5 5′-GGAAGTAAAAGTCGTAACAAGG
ITS 4 5′-TCCTCCGCTTATTGATATGC
benA - β-tubulin  1125 β-tub 2a 5′-GGTAACCAAATCGGTGCTGCTTTC
β-tub 2b 5′-ACCCTCAGTGTAGTGACCCTTGGC
cmdA Calmodulin 543 Cmd5 5′-CCGAGTACAAGGAGGCCTTC-3′
Cmd6 5′-CCGATAGAGGTCATAACGTGG-3′