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. 2019 Mar 27;9:5257. doi: 10.1038/s41598-019-41727-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The ZIKV-infected F₀ generation of Cx. quinquefasciatus mosquitoes at 7 days postinfection. The mosquitoes were infected via artificial blood feeding. Positive test results for the presence of the ZIKV antigen by using ICC staining of the positive head (A), negative head (B), positive salivary glands (C), negative salivary glands (D), positive midguts (E), negative midguts (F), positive yoke granules (G), negative yoke granules (H), positive facet cells (I) and negative facet cells (J).