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. 2019 Mar 27;9:5239. doi: 10.1038/s41598-019-41754-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Temperature–dependence of starvation survival time. (A,B) The relationship between temperature and starvation survival time in Oregon R (A), and Canton S flies (B) is best described by an exponential function. Starvation survival time was measured in four-day old males that were transferred to 1% agarose (in distilled water) and exposed to eleven different temperatures. Lines represent exponential regressions. Each data point represents the mean value ± s.e.m. Sample size is reported in parenthesis. (C,D) The relationship between inverse temperature and the starvation survival time in Oregon R (C), and Canton S flies (D) is best described by a linear function. Lines represent linear regressions. (E,F) Plot of residuals vs inverse temperature in Oregon R (E), and Canton S flies (F). The residuals did not depart from 0 in a systemic manner (Shapiro-Wilk W test of normality: Oregon R: P-value = 0.08; Canton S: P-value = 0.99).