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. 2019 Mar 27;10:1388. doi: 10.1038/s41467-019-09374-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Overview of targeted RNAseq and panel validation. a Schematic of targeted RNAseq process. b Scatterplot of targeted RNAseq enrichment for ERCCs included on (blue) or excluded from (orange) the blood panel. c Abundance of captured ERCCs before and after targeted sequencing on blood panel. d Metagene plot of K562 targeted RNAseq read coverage across all genes on the blood panel. TS=Transcript Start site; TE=Transcript End site