Fig. 4.

Fusion junction diversity and gene expression. a Schematic of BCR-ABL1 fusion isoforms +/−BCR exon 14. b TMPRSS2 and ERG gene structures and TMPRSS2-ERG fusion isoform prevalence. Bar charts on the right indicate the number of samples expressing each isoform. For simplicity, junctions beyond exon 1 are depicted utilising exon 1a. Black line represents retained intronic sequence. c, d Schematic of EZR-ROS1 and ACLS3-ETV1 fusions and quantification of read count expression across the endogenous genes in lung cancer sample MO-16-000393 and prostate cancer sample 12543, respectively. Horizontal lines indicate mean expression levels; coloured dots represent expression from the fused alleles plus nonrearranged alleles, while the grey dots represent expression of the canonical, nonrearranged alleles