Figure 5.

Tesra occupancy at the Prss/Tessp locus. ChIRP-qPCR for Tesra in mouse testicular germ cells was performed. Germ cells were collected from 21- to 22-day-old mouse testes, and nuclear extracts were prepared. Sonicated chromatin was hybridized with biotinylated tiling oligo probes, and the bound chromatin was collected by streptavidin beads. Purified genome DNAs were investigated by qPCR. Rec8 and B2m promoters that were located on different chromosomes from the Prss/Tessp cluster were amplified as negative controls. The value was normalized to that of the input sample, which was kept before hybridization, and further normalized to the level at the Rec8 promoter (=1.0). The relative chromatin enrichment at each position is shown by the black bars. The white bars show the data from the experiment with RNase in hybridization buffer as a negative control. Positions of amplicons are indicated by arrows below the graph. The data are presented as means ± SD from three independent experiments and were analyzed by one-way ANOVA followed by the Tukey post hoc test. *P < 0.05 compared to other regions.