Figure 5.

Acute effects of semen components on semi-purified endometrial epithelial cell gene expression. Semi-purified endometrial epithelial cell expression of CSF2, IL6, CXCL8, IL17A, TGFB1, IFNE, PTGS2, AKR1C4 OXTR, and IL1B in response to 5% seminal plasma (SP) or vehicle medium alone (vehicle). Gene expression was measured in terminal experiments starting at 15 min, up to 120 min. Data are presented as the mean relative expression + SEM from six independent experiments. Data were log transformed and analyzed using the generalized linear mixed model with pairwise comparisons made with vehicle treated control within each time point. *, P value of ≤ 0.05. T, treatment effect.