TABLE 3.
MIC of E. coli TOP10 strains expressing native OXA-48, OXA-48:P68A, and OXA-48:P68A,Y211S
| Antimicrobial agenta | MIC (mg/liter)b
|
|||
|---|---|---|---|---|
| TOP10 | MP104 | MP105 | MP106 | |
| Penicillins and inhibitor combinations |
||||
| TRM | 16 | 256 | 64 | 32 |
| TZP | 2 | 64 | 2 | 2 |
| AMC | 4 | 128 | 128 | 64 |
| Cephalosporins | ||||
| CAZ | 1 | 0.5 | 16 | 8 |
| CAZ-AVI | 0.25 | 0.25 | 0.25 | 1 |
| CXM | 16 | 16 | 16 | 16 |
| FEP | 0.06 | 0.12 | 0.25 | 0.12 |
| FOT | 0.12 | 0.25 | 0.25 | 0.12 |
| Carbapenems | ||||
| MEM | 0.03 | 0.25 | 0.03 | 0.03 |
| IMI | 0.25 | 1 | 0.25 | 0.25 |
| ETP | 0.015 | 1 | 0.06 | 0.06 |
| DOR | 0.03 | 0.03 | 0.03 | 0.03 |
a
TRM, temocillin; TZP, piperacillin-tazobactam with tazobactam fixed at 4 µg/ml; AMC, amoxicillin-clavulanic acid with clavulanic acid fixed at 2 µg/ml; CAZ, ceftazidime; CAZ-AVI, ceftazidime-avibactam, with avibactam fixed at 4 µg/ml; CXM, cefuroxime; FEP, cefepime; FOT, cefotaxime; MEM, meropenem; IMI, imipenem; ETP, ertapenem; DOR, doripenem.
b
Shown are the MICs of E. coli TOP10 and corresponding strains expressing native OXA-48, OXA-48:P68A, and OXA-48:P68A,Y211S (MP104, MP105 and MP106, respectively). For expression, genes were subcloned into the pCR-blunt II TOPO vector. Tests were performed in duplicates.