Figure 2.

The original bright-field (A) and polarized light microscopy images (D); Grayscale representation of the green layer isolated from RGB composition and segmented from bright-field image (B), and from polarized image (E). (C) The segmented images from (B) were subjected to a cycle of erosion, dilation, and erosion to remove the pixel associated with the media while maintaining image integrity. Isolated islands were also removed to remove extraneous debris. (F) A visual representation of the polarized image separated by adventitia (green) and the media (white). The scale bar is 200 µm.