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Photomicrographs of paired, stained tissue samples with (left column) and without (right column) HIER. The images show strong immunostaining even without HIER. The immunostains include PR 1294 (Panels A and B), HER2 SP3 (Panels C and D), HercepTest (Panels E and F), and PR 636 (Panels G and H). Scale bar, 20 µm. Abbreviations: HIER, heat-induced epitope retrieval; PR, progesterone receptor; HER2, human epidermal growth factor receptor 2.
