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Staining of tissue sections using the ER SP1 immunostain, with (A and C) and without HIER (B and D). Panels A and B depict a strongly staining carcinoma with almost equal stain intensity regardless of whether HIER was used. Panels C (with HIER) and D (without HIER), on the contrary, illustrate a significant difference in stain intensity. Scale bar, 20 µm. Abbreviations: ER, estrogen receptor; HIER, heat-induced epitope retrieval.
