Table 1.

LC-MS/MS operating conditions to analyze various LPLs and PLs

Analyte	Mode	MS/MS				LC
		Precursor ion (m/z)	Product ion (m/z)	DP (v)	CE (v)	Mobile phase (A/B, v/v)		Column	Flow rate (mL/min)
						A	B
LPA C18:2	Neg	433	79	−46	−106	85	15	C18	0.25
LPA C16:0	Neg	409	79	−61	−86
LPA C18:1	Neg	435	79	−61	−72
LPA C14:0 (IS)	Neg	381	79	−66	−82
LPC C18:2	Pos	520	104	76	45	85	15	C18	0.2
LPC C14:0 (IS)	Pos	468	104	81	37
LPE C18:2	Neg	476	79	−61	−112	85	15	C18	0.25
LPE C16:0	Neg	452	79	−111	−108
LPE C18:1	Neg	478	79	−61	−112
LPI C18:2	Neg	595	79	−86	−114
LPI C16:0	Neg	571	79	−101	−124
LPI C18:1	Neg	597	79	−86	−114
LPA C14:0 (IS)	Neg	381	79	−66	−82
PA 16:0-18:2	Neg	671	79	−121	−130	90	10	HILIC	0.25
PA 18:2-18:2	Neg	695	79	−96	−128
PA 16:0-18:1	Neg	674	79	−76	−128
PA 14:0-14:0 (IS)	Neg	591	227	−86	−44
PC 18:2-18:2	Pos	782	184	116	49	80	20	HILIC	0.25
PC 16:0-18:2	Pos	758	184	116	45
PC 14:0-18:0 (IS)	Pos	735	184	136	37
PI 18:2-18:2	Neg	857	79	−141	−122	60	40	HILIC	0.25
PI 16:0-16:0	Neg	810	79	−141	−122
PA 14:0-14:0 (IS)	Neg	591	227	−86	−44

CE, collision energy; DP, declustering potential; HILIC, Hydrophilic Interaction; IS: internal standard; LC-MS/MS, liquid chromatography-tandem mass spectrometry; LPA, lysophosphatidic acid; LPC, lysophosphatidylcholine; LPE, lysophosphatidylethanolamine; LPI, lysophosphatidylinositol; LPL, lysophospholipid; PA, phosphatidic acid; PC, phosphatidylcholine; PI, phosphatidylinositol; PL, phospholipid.