Table 2.
Amounts of lipids in GEF
| Analyte | Calibration equation | R | Amount (mg/g) | Content (%) |
|---|---|---|---|---|
| Linoleic acid | y = 0.0156x − 0.4116 | 0.9929 (R2) | 75.28 ± 2.5 | 7.53 |
| Palmitic acid | y = 0.017x − 0.1685 | 0.9961 (R2) | 28.18 ± 0.8 | 2.82 |
| Oleic acid | y = 0.0163x − 0.4502 | 0.9910 (R2) | 14.64 ± 0.3 | 1.46 |
| LPA C18:2 | y = 1.38x + 0.00283 | 0.9995 | 2.01 ± 5.61 | 0.20 |
| LPA C16:0 | y = 2.25x − 0.00383 | 0.9999 | 0.57 ± 3.04 | 0.06 |
| LPA C18:1 | y = 1.83x − 0.000639 | 0.9994 | 0.19 ± 0.42 | 0.02 |
| LPC C18:2 | y = 1.81x − 0.0055 | 0.9999 | 0.82 ± 4.39 | 0.082 |
| LPE C18:21) | LPE 18:1 | — | 0.12 ± 8.56 | 0.012 |
| LPE C16:0 | y = 0.358x − 0.00058 | 0.9993 | 0.19 ± 2.22 | 0.019 |
| LPE C18:1 | y = 0.472x + 0.00063 | 0.9993 | BSL | BSL |
| LPI C18:21) | LPI 18:1 | — | 0.93 ± 3.01 | 0.093 |
| LPI C16:0 | y = 1.26x + 0.000244 | 0.9985 | 0.38 ± 4.17 | 0.038 |
| LPI C18:1 | y = 1.06x + 0.000765 | 0.9984 | 0.11 ± 6.25 | 0.011 |
| PA 16:0-18:2 | y = 0.00636x2 + 0.909x + 0.0159 | 0.9992 | 11.16 ± 0.95 | 1.12 |
| PA 18:2-18:2 | y = −0.00322x2 + 0.981x + 0.0258 | 0.9999 | 4.84 ± 5.16 | 0.48 |
| PA 16:0-18:1 | y = 0.000148x2 + 1.15x − 0.000798 | 0.9990 | 1.53 ± 4.96 | 0.15 |
| PC 18:2-18:2 | y = 1.79x2 + 8.04x − 0.0112 | 0.9992 | 0.23 ± 1.32 | 0.023 |
| PC 16:0-18:2 | y = 2.66x2 + 7.33x − 0.0026 | 1.0000 | 0.28 ± 1.47 | 0.028 |
| PI 18:2-18:21) | PI 16:0-16:0 | — | BSL | BSL |
| PI 16:0-16:0 | y = −0.00318x2 + 1.13x − 0.0317 | 0.9984 | BSL | BSL |
Amount (mg/g) = mean ± RSD (%) from three different samples of white ginseng.
BSL, below the sensitivity limit; GEF, gintonin-enriched fraction; LPA, lysophosphatidic acid; LC-MS/MS, liquid chromatography-tandem mass spectrometry; LPC, lysophosphatidylcholine; LPE, lysophosphatidylethanolamine; LPI, lysophosphatidylinositol; MRM, multiple reaction monitoring; PA, phosphatidic acid; PC, phosphatidylcholine; PI, phosphatidylinositol; RSD, relative standard deviation.
The amount of LPE C18:2, LPI C18:2, and PI 18:2-18:2 was determined without standards, and were identified and quantified using MRM transitions. The methods of LC-MS/MS conditions and calibration equations were obtained by LPE C18:1, LPI C18:1, and PI 16:0-16:0.