Fig. 1.
Ginsenoside Rg3 (G-Rg3) inhibited histamine production from activated mast cells. (A) HMC-1 cells (1 × 104 cells/well) were treated with G-Rg3 (2-50 μg/mL) for 24 h. (B) RBL-2H3 cells (5 × 103 cells/well) were treated with G-Rg3 (2-50 μg/mL) for 24 h. Cell viability was determined by MTT assay and WST assay, respectively. The production level of histamine was measured using the ELISA kit. (C) HMC-1 cells (2 × 105 cells/well) were pretreated with G-Rg3 and PDN (10 μM) for 30 min and stimulated with PMA (50 nM) + A23187 (1 μM) for 24 h. (D) DNP-IgE (100 ng/mL)–sensitized RBL-2H3 cells (2 × 105 cells/well) were pretreated with G-Rg3 and PDN (10 μM) for 30 min and stimulated with DNP-BSA (10 μg/mL) for 24 h. Results are the mean ± SD. #p < 0.05 versus the blank; *p < 0.05, **p < 0.01, and ***p < 0.001 versus stimulator-treated group.
ELISA, enzyme-linked immunosorbent assay; PDN, prednisolone; PMA, phorbol 12-myristate 13-acetate; SD, standard deviation.