Figure 5.

The opsonic killing activity of four two fold dilutions of specific anti-FLA and anti-FLB IgG against Pseudomonas aeruginosa strains PAK (A) and PAO1 (B). Each strain was separately incubated with specific antisera raised against FLA and FLB, and mouse macrophage in the presence of rabbit complement. Significant opsonophagocytosis was observed when the specific antiserum was treated with the homologous strain. It seems that the most cross-reaction was occurred when the anti-FLA IgG was treated with heterologous strain PAO1. Bars represent means of duplicate determinations, and error bars indicate SD. Three asterisks represent P<0.001