FIG 2.

CF-301 synergizes with human blood components against S. aureus strain MW2. (A) Sensitivity of the synergistic effect to proteinase K. MIC determinations were performed in the presence of alamarBlue, a dye that enabled an assessment of bacterial viability (pink) and death (blue) over the indicated dilution range. (B) Temperature sensitivity of the synergistic effect. (C) Checkerboard analysis of CF-301 and human serum in caMHB. The red squares indicate combinations with diminished synergy. (D and E) Time-kill analysis of CF-301 with a concentration range of HuLYZ (in micrograms per milliliter) and HSA (in milligrams per milliliter), respectively, in CAMHB/HiHuS. Mean values (±SEM) are indicated for three independent technical replicates. (F) Lytic assays examining HuLYZ and HSA without CF-301. (G and H) Lytic activities of 4 μg/ml CF-301 alone and with a concentration range of HuLYZ (in micrograms per milliliter) and HSA (in milligrams per milliliter), respectively. (I) Lytic assays examining CF-301 with HuLYZ and HSA.