TABLE 3.
Molecular epidemiology and resistance mechanism results for isolates included in this study
| Species | Isolate no. | Yr | MLSTa | Country | Lefamulin MIC (μg/ml) | Resistance determinantsb |
Ribosomal mutationsc |
||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Pleuromutilins | Other | 23S rRNA | L3 | L4 | L22 | ||||||
| S. aureus | 975498 | 2016 | 5 | United States | 0.5 | erm(A) | A21G, A1557T | WT | WT | WT | |
| S. aureus | 981256 | 2016 | 4335 | New Zealand | 0.5 | vga(A) | A21G, A1557T, A2234G | WT | WT | WT | |
| S. aureus | 924825 | 2015 | 88 | Australia | 1 | vga(A) | erm(C) | A21G, A1557T, A2234G | WT | WT | WT |
| S. aureus | 953474 | 2016 | 398 | France | 1 | vga(A) | erm(T) | A21G, A1557T, A2234G | WT | WT | WT |
| S. aureus | 879822 | 2015 | 1 | Slovenia | 2 | vga(A) | A21G, A2234G | WT | WT | WT | |
| S. aureus | 913640 | 2015 | 1148 | United States | 2 | vga(A) | erm(C) | A21G, A2234G | WT | WT | WT |
| S. aureus | 934242 | 2016 | 1148 | United States | 2 | vga(A) | A21G, A2234G | WT | WT | WT | |
| S. aureus | 950457 | 2016 | 97 | United States | 4 | vga(A) | A21G, A2234G | WT | WT | WT | |
| S. aureus | 916083 | 2015 | 5 | Korea | 16 | erm(A) | A21G, A1557T, A2234G | WT | V118A, E147K | WT | |
| S. aureus | 976441 | 2016 | 398 | Brazil | 32 | lsa(E) | erm(T), lnu(B) | A21G, A1557T, A2234G | WT | WT | WT |
| S. aureus | 972481 | 2016 | 398 | Germany | >32 | vga(E) | erm(A) | A21G, A1526G, A1557T | WT | WT | WT |
| S. cohnii | 939671 | 2016 | N/A | United States | 0.5 | msr(A) | A107G, A124G, T266G, C450T, T623C, A816G, T1261C, T1448A, T1549A | D108E, T190A, N193K, Y208F | N20S, A128T, A133T, V155I | WT | |
| S. epidermidis | 939504 | 2016 | 2 | Italy | 0.5 | msr(A) | G105A, G241T, T669C, T1236C, G2576T | WT | WT | WT | |
| S. epidermidis | 947675 | 2016 | 57 | United States | 1 | vga(A) | mph(C), msr(A) | G241T, T669C, T1236C | WT | WT | WT |
| S. epidermidis | 951555 | 2016 | 87 | Czech Republic | 1 | vga(A), vga(B) | erm(A) | C139T, G241T, T669C, T1236C | WT | WT | WT |
| S. epidermidis | 955639 | 2016 | 87 | Italy | 1 | vga(A), vga(B) | vat(B) | C139T, T669C, T1236C, C2809T | WT | WT | WT |
| S. epidermidis | 956923 | 2016 | 679 | Brazil | 2 | vga(A) | msr(A) | T669C, T1236C | V188I | WT | WT |
| S. epidermidis | 949426 | 2016 | 255 | United States | 2 | vga(A) | T669C, T1236C | WT | WT | WT | |
| S. epidermidis | 938399 | 2016 | 5 | United States | 8 | vga(A) | mph(C), msr(A) | C139T, T669C, T1236C | WT | WT | WT |
| S. epidermidis | 952506 | 2016 | 20 | Argentina | 8 | vga(A) | mph(C), msr(A) | C139T, T669C, T1236C, C1638T | WT | WT | WT |
| S. epidermidis | 958510 | 2016 | 487 | United States | 8 | vga(A) | G241T, T669C, T1236C, C1638T | WT | WT | WT | |
| S. epidermidis | 934123 | 2016 | 5 | United States | 32 | cfr | T669C, T1236C, C2534T | H146Q, V154L, A157R | G71_R72insG | WT | |
| S. haemolyticus | 939969 | 2016 | 3 | Mexico | 4 | cfr | mph(C), msr(A), erm(C) | C1486T, A2235G, T2882C | WT | WT | A29T |
| S. sciuri | 944662 | 2016 | N/A | Mexico | 16 | sal(A) | erm(C) | WT | WT | WT | A112D |
| S. sciuri | 941213 | 2016 | N/A | Australia | 32 | sal(A) | WT | WT | WT | WT | |
| S. lutetiensis | 960742 | 2016 | N/A | Belgium | 0.5 | lnu(C) | T225C, A2360G | WT | WT | WT | |
| S. lutetiensis | 982012 | 2016 | N/A | Argentina | 2 | lsa(E) | lnu(B), lnu(C) | WT | WT | WT | WT |
| S. agalactiae | 971459 | 2016 | 19 | Korea | 8 | lsa(E) | lnu(B), erm(B) | WT | WT | R1del, R2K | WT |
| S. agalactiae | 935557 | 2016 | 19 | Mexico | 8 | lsa(E) | lnu(B), erm(B) | WT | WT | R1del, R2K | WT |
| S. agalactiae | 935554 | 2016 | 19 | Mexico | 16 | lsa(E) | lnu(B), erm(B) | WT | WT | R1del, R2K | WT |
| S. gallolyticus | 965031 | 2016 | N/A | Spain | 32 | lsa(E) | lnu(B), erm(B) | C696T | WT | WT | WT |
a
MLST, multilocus sequence typing; N/A, not available.
b
MLSB (macrolide-lincosamide-streptogramin B) and pleuromutilin resistance genes screened as available at https://faculty.washington.edu/marilynr/ermwebA.pdf.
c
23S rRNA mutational analysis performed on nucleotide sequences (Escherichia coli numbering). Protein sequences analyzed for annotating L3, L4, and L22.