TABLE 2.
Mutational changes of genes conferring antibiotic resistance and their expression levels in PA154197 relative to that in PAO1a
| Gene | Nonsynonymous/indel mutation(s) in encoding region | Fold change (PA154197/PAO1) | Log2-fold change (PA154197/PAO1) |
|---|---|---|---|
| mexS | D249-N | 2.61 | 1.38 |
| mexT | Del (8 bp: 237–244), F172-I, G301-C | 1.52 | 0.60 |
| mexE | NA | 122.10 | 6.93 |
| mexF | NA | 97.54 | 6.61 |
| oprN | NA | 102.94 | 6.69 |
| mexG | NA | 12.55 | 3.65 |
| mexH | NA | 13.91 | 3.80 |
| mexI | A782-E, A936-T | 14.58 | 3.87 |
| opmD | NA | 13.64 | 3.77 |
| armR | S21-T, Y32-C | 2.87 | 1.52 |
| mexR | E76-* | 5.82 | 2.54 |
| mexA | NA | 11.08 | 3.47 |
| mexB | I1001-T | 11.49 | 3.52 |
| oprM | NA | 10.84 | 3.44 |
| mexX | Q4-H, K329-Q, L331-V, W358-R | — | — |
| mexY | T543-A, L984-F | — | — |
| mexZ | NA | — | — |
| mexC | P57-S, R76-Q, H310-R, S330-A, A378-T, P383-S, A384-V | 4.14 | 2.05 |
| mexD | T87-S, A155-T, E257-Q, E314-D, V434-A, N669-D, S685-G, I703-V, S845-A, S915-A, I982-V, K1031-R, S1040-T | 1.64 | 0.72 |
| oprJ | T376-S, K413-M | — | — |
| mexJ | F13-L, A314-P | 7.24 | 2.86 |
| mexK | I21-L, K694-R, A841-T | 8.93 | 3.16 |
| mexL | S4-P | 1.96 | 0.97 |
| muxA | T261-A | — | — |
| muxB | NA | — | — |
| muxC | NA | — | — |
| opmB | NA | — | — |
| mexP | NA | 2.05 | 1.03 |
| mexQ | I294-V, V384-I, G505-D, V517-A, R656-K, D722-E | — | — |
| opmE | S46-G, I50-V, S175-T, W354-R | — | — |
| oprD | D43-N, S57-E, S59-R, E202-Q, I210-A, E230-K, S240-T, N262-T, A267-S, A281-G, K296-Q, Q301-E, R310-G, V359-L, Del(6 bp:1132-1137), M372-V, S373-D, D374-S, N375-S, N376-S, V377-S, K380-Y, N381-A, Y382-G, G383-L | 0.64 | –0.65 |
| ampC | T105-A | 0.54 | –0.88 |
| ampR | NA | — | — |
| gyrA | T83-I, Del(6 bp: 2734–2739) | — | — |
| gyrB | NA | — | — |
| parC | NA | — | — |
| parE | NA | 1.79 | 0.84 |
| parS | H398R | 1.55 | 0.64 |
| parR | NA | 1.53 | 0.61 |
| mvaT | NA | 0.71 | –0.48 |
| poxB | NA | 0.17 | –2.55 |
| nalC | G71-E, E153-Q, S209-R | 1.33 | 0.42 |
| nalD | NA | — | — |
| aph | A14-S | — | — |
| amgS | I260-V, G288-D | — | — |
| spuF | P276-S | — | — |
| fusA1 | I186-V | 1.85 | 0.89 |
| fusA2 | S176-A, A690-S, G695-A | 0.15 | –2.78 |
a
NA, no nonsynonymous mutation occurred; Del, deletion; —, no significant difference; *, stop codon.