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. Author manuscript; available in PMC: 2019 Mar 28.
Published in final edited form as: Lab Invest. 2017 Oct 16;97(12):1412–1426. doi: 10.1038/labinvest.2017.96

Figure 3.

Figure 3.

Overexpression of myocardin promotes HSC myofibroblast differentiation and liver fibrosis. (A) A schematic map for overexpression of myocardin with 4 doses of CCl4. 5 days after the final dose of CCl4, HSCs were isolated and cultured overnight; representative phase contrast images are shown (arrow indicates an activated HSC). (B, C) Total RNA was extracted from the same cells and RT-PCR was performed to detect SM α-actin and Col.1α1 mRNA (n=3, p < 0.01 for Ad-control (Ad-ctr) vs. Ad-myocd). (D) A schematic map for overexpression of myocardin with 6 doses of CCl4. 5 days after the final CCl4 dose, whole liver tissues were harvested and subjected to picrosirius red staining as in Methods. Quantitative data is shown graphically (n=3 ~ 4, p < 0.05 for Ad-control (Ad-ctr) vs. Ad-myocd). The scale bar represents 100 μm.