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. 2019 Jan 25;22:21–36. doi: 10.1016/j.molmet.2019.01.006

Figure 1.

Figure 1

RNA-Seq of preadipocytes revealed depot-specific signatures and MME as a highly differentially expressed gene between visceral and subcutaneous preadipocytes. Human subcutaneous, omental, and mesenteric preadipocyte primary cultures were grown to confluency, harvested for RNA, and subjected to RNA-seq to generate gene expression profiles. For mouse gene expression profiles, inguinal and epididymal preadipocyte primary cultures were grown to confluency, harvested for RNA, and subjected microarray analysis. (A) Principal component analysis of human subcutaneous, mesenteric, and omental gene expression profiles shows clustering by depot. (B) Principal component analysis of inguinal and epididymal gene expression profiles shows clustering by depot. (C) Differential gene expression followed by Gene Set Enrichment Analysis (GSEA) revealed a set of pathways differentially expressed between subcutaneous and visceral preadipocytes in human and mouse tissue. SC = abdominal subcutaneous. MES = Mesenteric. OM = Omental. Epi = Epididymal. SubQ = Inguinal. N = 3–5. (D) The 20 significant pathways were analyzed to reveal genes found in multiple pathways. A total of four genes (ABAT, ITGB8, MME, and F11R) were significant in multiple pathways. (E) RNA-seq expression of Membrane Metallo-Endopeptidase (MME) in subcutaneous and visceral preadipocytes (Omental = OM, Mesenteric = MES). (F) Microarray expression of MME in mouse subcutaneous (SubQ) and visceral preadipocytes (Epi). Asterisks indicate p ≤ 0.05 by moderated t-test (limma). N = 3–5. Bars indicate mean ± s.e.m.