Figure 8.

MME does not form a protein complex with the insulin receptor. Immortalized IR/IGFR double-knockout murine brown preadipocytes were transiently transduced with either pCMV-MME alone or pCMV-MME and pCMV-IR-3XFLAG for 48 h before protein isolation and immunoprecipitation followed by western blot. (A) Western blot of protein lysate and the immunoprecipitation of IR-FLAG in IR/IGFR double-knockout brown preadipocytes in serum-fed media. N = 2 (B) Protein-protein interaction network between MME and INSR. The shortest paths in between MME (bottom node) and INSR (top node) was determined in the PPI as defined in BioGRID. RNA-seq from subcutaneous adipose tissue from ENCODE was plotted on the nodes. One possible path is INSR-CAV1-FLOT2-MME, which are proteins found on endocytic vesicles.