Table 1.
Pleiades number | Gene | MiniPromoter size (bp) | PlasmidpEMS numbera | MiniPromoter design type | Figure showing expression |
---|---|---|---|---|---|
Ple320 | POU4F1 | 1,969 | 2241 | New | 2, 3, 4, S2 |
Ple321 | TUBB3 | 1,829 | 2242 | New | 2, 4, 5, S2 |
Ple342 | TUBB3 | 1,992 | 2272 | New | 2, S2 |
Ple343 | TUBB3 | 2,669 | 2273 | New | 2, S2 |
Ple344 | TUBB3 | 801 | 2274 | Cutdown of Ple321 | 2, 3, 4, 5, S2 |
Ple345 | NEFL | 2,693 | 2280 | New | 2, 3, 4, 5, 6, 7, S2 |
Ple346 | NEFM | 2,771 | 2281 | New | 2, 4, S2 |
Plasmid constructs available from Addgene.
NEFL; neurofilament, light polypeptide; NEFM; neurofilament, medium polypeptide; Ple, pleiades MiniPromoter drove emerald green fluorescent protein–woodchuck hepatitis virus post-transcriptional regulatory element; POU4F1, POU domain, class 4, transcription factor 1; TUBB3; tubulin, beta 3 class III; pEMS, plasmid.