Table 1. Comparison of Different Methods to Image Single Intracellular fNDs Including a Qualitative Assessment on Their Performance.

technique	specificity for fND clusters	specificity for single NDs	resolution	fluorescence (FL)
CLEM	high specificity depending on the fND emission	low specificity	high resolution (EM grade)	FL information from both fNDs and labeled organelles
EFTEM	high specificity depending on the fND unique energy absorption	high specificity depending on the unique energy adsorption of fNDs	high resolution (EM grade)	no FL information
HAADF STEM	medium specificity depending on the fND orientation	medium specificity depending on the fND orientation	high resolution (EM grade)	no FL information
TEM (overfocus)	medium specificity depending on the fND Fresnel contrast fringes	medium specificity depending on the fND Fresnel contrast fringes	high resolution (EM grade)	no FL information