Figure 4.

PEX5 ablation in β-cells affects the expression of genes associated with apoptosis. RNA-seq was performed on islets of HFD-fed Rip-Pex5^−/− (n = 3) and control mice (n = 4). (A) Principal component analysis and (B) Volcano plot representing the RNA-seq expression data from all biological replicates of Rip-Pex5^−/− and control mice. In the Volcano plot, blue and red indicate, respectively, the up- and down-regulated genes in Rip-Pex5^−/− mice compared with control mice. (C) Heat map of differentially expressed genes (fold change > 2; p-value> 0.05) in β-cells of Rip-Pex5^−/− mice as compared to control β-cells. Lower and higher levels of expression are presented in shades of blue and orange, respectively. (D) GSEA against the KEGG database showing enriched signaling pathways in β-cells of Rip-Pex5^−/− mice.