Figure 3.

Effects of AO-1 on the infiltration of CD4⁺, CD8⁺ T cells, and CD11b⁺ monocytes in the spinal cord of EAE mice. (a) IHC analysis of untreated vehicle and AO-1-treated groups. (b)–(d) Quantitation of CD4⁺, CD8⁺ T cells, and CD11b⁺ monocytes. On Day 21 after MOG_35–55 immunization, the infiltrated T cells and monocytes in the spinal cord of EAE mice were measured through IHC analysis using specific antibodies. The numbers of CD4⁺, CD8⁺ T cells, and CD11b⁺ monocytes were quantified. Data are presented as mean ± SEM (n = 4). ∗ P < 0.05 and ∗∗∗ P < 0.001 compared with vehicle group. Scale bars, 50 μm.