(a) Workflow to assess effects of ZIP transporter overexpression on 70Zn(II) uptake. (b) HEK293T cells transfected with ZIP4, ZIP8, or ZIP10 took up significantly more 70Zn(II) over a 2-h period than did cells transfected with GFP (N = 3, ±SEM). (c) HEK293T cells transfected with ZIP4, ZIP8, or ZIP10 contained significantly more total Zn(II) than did cells expressing GFP (N = 3, ±SEM). p-values indicate Student’s t-test adjusted with the Bonferroni correction for multiple testing (*** indicates p < 0.001, ** indicates p < 0.01).