KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Bacterial and Virus Strains | ||
| Biological Samples | ||
| Chemicals, Peptides, and Recombinant Proteins | ||
| Tetrodotoxin | Sigma-Aldrich | 554412; CAS 18660–81–6 |
| Critical Commercial Assays | ||
| Deposited Data | ||
| Experimental Models: Cell Lines | ||
| Experimental Models: Organisms/Strains | ||
| Drosophila: 13XLexAop2-IVS-Syn21-OpGCaMP6s-p10 (su(Hw)attP5) | Rubin Lab, JRC, HHMI | N/A |
| Drosophila: 13XLexAop2-IVS-Syn21-OpGCaMP6s-p10 (su(Hw)attP8) | Rubin Lab, JRC, HHMI | N/A |
| Drosophila: 20XUAS-iGluSnFR.A184V (attP2) | Bloomington | 59611 |
| Drosophila: 20XUAS-IVS-NES-jRCaMP1b-p10 (VK00005) | Bloomington | 63793 |
| Drosophila: 20XUAS-IVS-Syn21-opGCaMP6s-p10 (su(Hw)attP1) | Rubin Lab, JRC, HHMI | N/A |
| Drosophila: 20XUAS-IVS-Syn21-opGCaMP6s-p10 (su(Hw)attP5) | Rubin Lab, JRC, HHMI | N/A |
| Drosophila: 20XUAS-IVS-Syn21-opGCaMP6s-p10 (su(Hw)attP8) | Rubin Lab, JRC, HHMI | N/A |
| Drosophila: 6–60–GAL4 (Chr 3) |
Ulrike Heberlein, JRC, HHMI; Nern et al., 2008 | N/A |
| Drosophila: alrm-Gal4 (Chr 2) | Bloomington | 67031 |
| Drosophila: alrm-Gal4 (Chr 3) | Bloomington | 67032 |
| Drosophila: brp(RSRT.Stop)V5–2A–LexA–VP16 (VK00003b) | This study (see Chen et al., 2014) | N/A |
| Drosophila: cn[1], bw[1] | Bloomington | 264 |
| Drosophila: Dm3-Gal4 [R25F07-GAL4 (attP2)] | Bloomington | 49130 |
| Drosophila: Dm4-Gal4 [R75F06-GAL4 (attP2)] | Bloomington | 39901 |
| Drosophila: Dm4-LexA [R75F06-LexA (attP40)] | Bloomington | 54100 |
| Drosophila: Dm9–1–Gal4 [VT025981.GAL4 (attP2)] | VDRC | 205425 |
| Drosophila: Dm9–2–Gal4 [R56G04-GAL4 (attP2)] | Bloomington | 39161 |
| Drosophila: Hdc[JK910] | Bloomington | 64203 |
| Drosophila: Hdc[MB07212] | Bloomington | 25260 |
| Drosophila: L3–1–Gal4 [R29F12-GAL4 (attP2)] | Bloomington | 49495 |
| Drosophila: L3–2–Gal4 [R9D03-GAL4 (attP2)] | Bloomington | 40726 |
| Drosophila: Mi1-LexA [R89C04-LexA (attp40)] | Bloomington | 54382 |
| Drosophila: Mi1-SplitGal4 [w1118; R55C05-p65ADZp (attP40); R71D01-ZpGdbd (attP2)] | Strother et al., 2017 (available from http://splitgal4.janelia.org) |
JRC_SS00955 |
| Drosophila: Mi4-Gal4 [R49B06-GAL4 (attP2)] | Bloomington | 50409 |
| Drosophila: Mi4-LexA [R49B06-LexA (attP40)] | Bloomington | 52707 |
| Drosophila: ort[1] | Bloomington | 1133 |
| Drosophila: PanLamina-1-FLP1 [27G05-FLP1 (su(Hw)attP5)] | This study (see Pecot et al., 2013) | N/A |
| Drosophila: PanLamina-2-FLP1 [27G05-FLP1 (Chr X)] | Tadros et al., 2016 | N/A |
| Drosophila: PanNeuronal-Gal4 [R57C10-Gal4 (attP2)] | Bloomington | 39171 |
| Drosophila: PanNeuronal-LexA [R57C10-lexA (attP40)] | Bloomington | 52817 |
| Drosophila: R7-GAL4 [sevEP-GAL4.B (Chr 2)] | Bloomington | 5793 |
| Drosophila: sens-R.pest (su(Hw)attP8) | This study (see Akin and Zipursky, 2016) | N/A |
| Drosophila: svp-Gal4 [svpNP0724(87B5)] | Kyoto DGGR | 103727 |
| Drosophila: T4/T5-Gal4 [R23G12-GAL4 (attP2)] | Bloomington | 49044 |
| Drosophila: T4/T5-LexA [R23G12-LexA (su(Hw)attP8)] | Bloomington | 65044 |
| Drosophila: Tm3-Gal4 [R13E12-GAL4 (attP2)] | Bloomington | 48569 |
| Drosophila: Tm3-LexA [R13E12-LexA (attP40)] | Bloomington | 48569 |
| Drosophila: Tm4-LexA [R35H01-LexA (attp40)] | Bloomington | 52459 |
| Drosophila: Tm9–1–Gal4 [R24C08-GAL4 (attP2)] | Bloomington | 48050 |
| Drosophila: Tm9–2–Gal4 [R25F10-GAL4 (attP2)] | Bloomington | 49132 |
| Drosophila: tubP-(FRT.GAL80) (attP40) | Rubin Lab, JRC, HHMI | N/A |
| Drosophila: UAS-ArcLight (attP2) | Bloomington | 51056 |
| Drosophila: UAS-CD-tdTOM (VK00033) | Bloomington | 35837 |
| Drosophila: UAS-TeTxLC.tnt (Chr 2) | Bloomington | 28838 |
| Drosophila: UAS-TeTxLC.tnt (Chr X) | Bloomington | 28996 |
| Drosophila: w[*] norpA[36] | Bloomington | 9048 |
| Oligonucleotides | ||
| Recombinant DNA | ||
| Software and Algorithms | ||
| Other | ||