Skip to main content
. Author manuscript; available in PMC: 2019 Jun 5.
Published in final edited form as: Sci Signal. 2018 Jun 5;11(533):eaar1976. doi: 10.1126/scisignal.aar1976

Fig. 4. Overexpression of Vap33 or knockdown of the V-ATPase component Vha55 suppresses the NEXT overexpression phenotype.

Fig. 4.

(A to E) Lateral views of the adult thorax of flies expressing the indicated transgenes in flies expressing RFP under the control of the eq> driver: (A) Control eq>RFP, lacZ; (B) eq>RFP, Vap33RNAi; (C) eq>RFP, Vap33o/e; (D) eq>RFP, Vha55RNAi; (E) eq>RFP, Vha44o/e. Arrowheads indicate the notum. (F to J) Lateral views of the adult thorax of flies expressing the indicated transgenes in the eq>NEXT background: (F) eq>NEXT, lacZ; (G) eq>NEXT, Vap33RNAi; (H) eq>NEXT, Vap33o/e; (I) eq>NEXT, Vha55RNAi; (J) eq>NEXT, mamDN. (K) Quantification of the thoracic area in all genotypes in (A) to (J). n = 2 independent experiments, n = 3 samples analyzed for each genotype per experiment. Error bars indicate SEM. ***P < 0.0001, *P = 0.01. ns, differences not significant (t tests with two-tailed distribution and equal variance or Mann-Whitney U test). Scale bars, 50 µm.