Table 2.
Oligonucleotides used for genotyping, sex determination and gene expression analysis in hsFLT1/rtTA mouse model.
| Gene | NCBI number | Primer sequence (5′ → 3′) | Product length (bp) |
|---|---|---|---|
| GENOTYPING | |||
| hsFLT1 | XM_017020485.1 | for: AATCATTCCGAAGCAAGGTG | 221 |
| rev: TTTCTTCCCACAGTCCCAAC | |||
| rtTA | for: AAAGTCGCTCTGAGTTGTTAT | ||
| rev-wt: GGAGCGGGAGAAATGGATATG | 650 | ||
| rev-mut: GCGAAGAGTTTGTCCTCAACC | 340 | ||
| SEX DETERMINATION | |||
| IL-3 | NM_010556.4 | for: GGGACTCCAAGCTTCAATCA | 544 |
| rev: TGGAGGAGGAAGAAAAGCAA | |||
| Sry | NM_011564.1 | for: TGGGACTGGTGACAATTGTC | 402 |
| rev: GAGTACAGGTGTGCAGCTCT | |||
| REFERENCE/HOUSEKEEPING GENE | |||
| Gapdh | XM_011241214.1 | for: ACAACTCACTCAAGATTGTCAGCA | 121 |
| rev: ATGGCATGGACTGTGGTCAT | |||
| AMINO ACID TRANSPORTERS | |||
| Slc38a1 | NM_001166458.1 | for: AGCACAGGCGACATTCTCATC | 133 |
| rev: ACAGGTGGAACTTTGTCTTCTTG | |||
| Slc38a2 | NM_175121.3 | for: ACAAATGGGTTGTGGTATCTG | 92 |
| rev: CCTAGATTTCTCAGCAGTGACAATG | |||
| FATTY ACID TRANSPORTERS | |||
| Cd36 | XM_006535623.2 | for: CAGTGCAGAAACAATGGTTGTCT | 137 |
| rev: TGACATTTGCAGGTCTATCTACG | |||
| Fabp3 | NM_010174.1 | for: CTGTCACCTCGTCGAACTCT | 166 |
| rev: TTTGTCGGTACCTGGAAGCT | |||
| GLUCOSE TRANSPORTERS | |||
| Glut-1 | NM_011400.3 | for: GCTGTGCTTATGGGCTTCTC | 202 |
| rev: ACACCTGGGCAATAAGGATG | |||
| Glut-3 | NM_011401.4 | for: GGAGGAGAACCCTGCATATGATA | 96 |
| rev: TGGCTTCATAGTCATCCTTTAGTAAC | |||
| LABYRINTHINE DIFFERENTIATION MARKERS | |||
| Cx26 | NM_008125.3 | for: ATGCTACGACCACCACTTCC | 194 |
| rev: TACGGACCTTCTGGGTTTTG | |||
| Gcm1 | NM_008103.3 | for: TGCTCACCTATGGCTCTCCT | 201 |
| rev: AAAATTCTGCCSAGCCCTTT | |||
| FETAL ENDOTHELIAL CELL MARKER | |||
| Cd31 | NM_008816.3 | for: ATGACCCAGCAACATTCACA | 200 |
| rev: CACAGAGCACCGAAGTACCA | |||
| TROPHOBLAST GIANT CELL MARKERS | |||
| Ctsq | NM_029636 | for: GTGATCTGAGGCAGTAGTGGTC | 180 |
| rev: GTACTTCTTCCTCCGGACTGTATA | |||
| PLAP | XM_006538500.2 | for: TGAGGGCAATGAGGTCACAT | 161 |
| rev: CCTCTGGTGGCATCTCCTTA | |||
| Prl3d1 | NM_008864 | for: TGGAGCCTACATTGTGGTGGA | 131 |
| rev: TGGCAGTTGGTTTGGAGGA | |||
| Prl3b1 | NM_008865.3 | for: AGCAGCCTTCTGGTGTTGTC | 197 |
| rev: TGTGACACCACAATCACACG | |||
| Prl2c2 | NM_011118 | for: AGGAGCCATGATTTTGGATG | 203 |
| rev: ACCAGGCAGGGTTCTTCTTT | |||
| SPONGIOTROPHOBLAST AND GLYCOGEN CELL MARKERS | |||
| Cx31 | NM_008126 | for: GTCTACTAGCGCTGGGATGG | 227 |
| rev: GTGCCAAACCTTCTCATGGT | |||
| Cx31.1 | NM_008126 | for: CCCTCTTTGCTTGTGGTCAT | 151 |
| rev: CCTTGAACGAGAGGCTGAAG | |||
| Igf2 | NM_010514 | for: CGTTTGGCCTCTCTGAACTC | 155 |
| rev: GACGACTTCCCCAGATACCC | |||
| Pcdh12 | NM_017378.2 | for: CTTCACCTCATCACGCTCAA | 197 |
| rev: TGCCCTCTGTCCTCTGCTAT | |||
| Tpbpa | NM_009411 | for: CCAGCACAGCTTTGGACATCA | 116 |
| rev: AGCATCCAACTGCGCTTCA | |||
| ANGIOGENESIS MARKERS | |||
| Flk-1 | NM_001363216.1 | for: GGCGGTGGTGACAGTATCTT | 162 |
| rev: GTCACTGACAGAGGCGATGA | |||
| m(s)Flt-1 | NM_001363135.1 | for: TATAAGGCAGCGGATTGACC | 159 |
| rev: TCATACACATGCACGGAGGT | |||
| Flt-4 | NM_008029.3 | for: GTGGCTGTGAAGATGCTGAA | 199 |
| rev: TGACACGCAAGAAGTTGGAG | |||
| Plgf | XM_011244016.1 | for: CGTCCTGTGTCCTTCTGAGT | 200 |
| rev: CCTCTTCCTCTTCCCCTTGG | |||
| Vegfa | NM_001025257.3 | for: CAGGCTGCTGTAACGATGAA | 140 |
| rev: GCATTCACATCTGCTGTGCT | |||
| Vegfb | NM_011697.3 | for: AACACAGCCAATGTGAATGC | 157 |
| rev: GGAGTGGGATGGATGATGTC | |||
| Vegfc | NM_009506.2 | for: CAAGGCTTTTGAAGGCAAAG | 159 |
| rev: TCCCCTGTCCTGGTATTGAG | |||
| Vegfd | NM_001308489.1 | for: CAACAGATCCGAGCAGCTTC | 155 |
| rev: AAAGTTGCCGCAAATCTGGT | |||
| PROAPOPTOTIC MARKERS | |||
| Bad | NM_001285453.1 | for: GGAGCTTAGCCCTTTTCGAG | 166 |
| rev: GCTTTGTCGCATCTGTGTTG | |||
| Casp9 | NM_001355176.1 | for: GATGCTGTCCCCTATCAGGA | 151 |
| rev: CGATGTACCAGGAGCCACTT | |||
| HYPOXIA-INDUCIBLE MARKERS | |||
| Nos3 | XM_006535639.3 | for: GACCCTCACCGCTACAACAT | 209 |
| rev: CTGGCCTTCTGCTCATTTTC | |||
| Hif1α | NM_001313920.1 | for: TCAAGTCAGCAACGTGGAAG | 198 |
| rev: TATCGAGGCTGTGTCGACTG | |||
| Hmox1 | NM_010442.2 | for: CACGCATATACCCGCTACCT | 175 |
| rev: CCAGAGTGTTCATTCGAGCA | |||
| Cited2 | NM_010828 | for: CTAGGGCAGCGGAGGAAAAG | 176 |
| rev: TTCTGCTCGGAACACCGAAG | |||
A, adenine; Bad, Bcl-2–associated death promoter; bp, base pair; Casp9, caspase 9; Cd31, cluster of differentiation 31; Cited2, Cbp/P300 Interacting Transactivator With Glu/Asp Rich Carboxy-Terminal Domain 2; Ctsq, cathepsin Q; C, cytosine; Cx26, connexin 26; Fabp3, fatty acid binding protein 3; Flk-1, fetal liver kinase 1; Flt-4, Fms-like tyrosine kinase 4; for, forward; Gapdh, glyceraldehyde-3-phosphate dehydrogenase; Gcm1, glial cell missing 1; Glut-1, glucose transporter 1; G, guanine; Hif1α, hypoxia-inducible factor-1alpha; Hmox1, Heme Oxygenase 1; hsFLT1, human soluble fms-like tyrosine kinase 1; Igf2, insulin-like growth factor 2; IL-3, interleukin-3; mut, mutant; NCBI, National Center for Biotechnology Information; Nos3, Nitric Oxide Synthase 3; Pcdh12, Protocadherin 12; PLAP, Placental Alkaline Phosphatase; Plgf, Placental Growth Factor; Prl3b1, prolactin family 3; subfamily b, member 1; rev, reverse; rtTA, reverse tetracycline-controlled transactivator; Slc38a1, Solute Carrier Family 38 Member 1; Sry, sex determining region Y; Tpbpa, Trophoblast-specific protein alpha; T, thymine; Vegfa, Vascular Endothelial Growth Factor A; wt, wild-type.