Fig. 2.

Elevated TRIM44 promotes melanoma cell progression both in vitro and in vivo. a, mRNA and protein levels of A2058, A875, MV3, M14, A375 cells. b, A2058 cells were transfected with short hairpin RNAs (shRNAs), while A375 cells were transfected with TRIM44. Transfection efficiencies were assessed by Western blot and qRT-PCR. c, Invasive abilities conveyed by A2058-shNC, A2058-shRNAs, A375-Vector, A375-TRIM44 were measured by Matrigel invasion assays. d, Migration ability conveyed by A2058-shNC, A2058-shRNAs, A375-Vector, A375-TRIM44 were assessed by wound-healing migration assays. e, Flow cytometric assay was used for detecting the apoptosis cells of A2058-shNC, A2058-shRNAs, A375-Vector, A375-TRIM44. f, Cell proliferation in A2058-shNC, A2058-shRNAs, A375-Vector, A375-TRIM44 cells was detected by CCK-8 assays. g, The in vivo effects of TRIM44 forced expression on lung metastasis were investigated by tail vein injection, and the number of metastases was examined by H&E staining. h, A375-TRIM44 and A375-Vector cells were subcutaneously inoculated in nude mice to test the proliferative ability in vivo. i, Tumor growth curves of the subcutaneous xenografts. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001