Fig. 1.

Up-regulation of FTO RNA demethylase in human breast cancer. a Heat map diagram of differential gene expression in breast tumors and normal tissues. b Expression of the m6A regulatory enzymes in primary human breast tumors. **P ≤ 0.01, ***P ≤ 0.001. c Relative FTO mRNA expression level in molecular subtypes and clinical stages of breast tumors. NORM: normal tissues; TNBC: ER−/PR−/Her2-; DNBC: ER−/PR−/Her2+; TPBC: ER+/PR+/Her2+. **P ≤ 0.01, ****P ≤ 0.0001. d Higher levels of FTO in human breast cancer tissues in comparison with normal breast tissues by immunohistochemistry assay. e FTO up-regulation was quantified from the immunohistochemistry results. f The global mRNA m6A level in human breast cancer samples determined by RNA m6A dot-blotting assay. g The global mRNA m6A level in human breast cancer samples determined by RNA m6A colorimetric analysis. *P ≤ 0.05. h FTO up-regulation was significantly associated with shorter overall survival in patients with advanced stage of breast cancer. i FTO up-regulation was significantly associated with shorter overall survival in patients with ER negative breast cancer