Figure 1.

Different applications and bioinformatics solutions for PacBio Iso-Seq and Nanopore direct RNA sequencing in plants. Iso-seq and direct RNA sequencing can be performed using the SMRT analysis from PacBio and MinKNOW from ONT, respectively. Then these long reads with FASTA format will undergo error correction step before downstream analysis. In order to deal with different applications (middle boxes), computational tools used to process and analyze long-reads for each application are indicated for species without and with reference genomes in left and right boxes, respectively.