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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: Biol Trace Elem Res. 2018 Sep 28;188(1):127–134. doi: 10.1007/s12011-018-1531-7

Fig. 2:

Fig. 2:

L1 worms were pre-treated for 30 min with 5 μM VU0063088 or VU0026921 and then exposed to 25 or 50 mM MnCl2 for 1 h. At least 60 worms per condition were observed under fluorescent microscope and scored for DAergic degeneration. (A) Depicts the head of a L1 worm with healthy neurons, scored as normal. (B) Neurons with puncta (discontinuous marking in the dendrite), loss or cell body shrinkage or loss of dendrites in their dopaminergic neurons were quantified as containing degeneration. Results are expressed as mean ± S.E.M. of 2 to 3 experiments with (C) Pdat-1::GFP worms or (D) smf-2, Pdat-1::GFP worms. * P <0.05 compared to vehicle (VEH) control group. Twoway ANOVA followed by post hoc Dunnet’s test. Representative images were obtained with a PerkinElmer spinning disk confocal, 60× objective. Scale bar represents 13 μm.