Fig. 3:
Basal slowing response (BSR) was evaluated in pdat-1::GFP (A) or smf-2, pdat-1::GFP worms (B) pre-treated for 30 min with 5 μM VU0063088 or VU0026921 and then exposed to 25 or 50 mM MnCl2 for 1 h. Controls were incubated with 85 mM NaCl. The test was performed 48 h after treatments. Worms were washed off plates and 5 worms were pipetted in test plates with or without OP-50 E. coli (food). After 5 min acclimation period, body bends were counted in 20 s intervals for each worm. BSR was expressed as number (average for all worms) of body bends off-food minus the number of body bends on food (Δ). Locomotion was expressed as the number of body bends/20 s on NGM plates without food (C) pdat-1::GFP, (D) smf-2, pdat-1::GFP. Data are expressed as mean ± S.E.M. of 3 to 4 experiments. * p <0.05, ** p <0.01 compared to the vehicle (VEH) control group. Two-way ANOVA followed by post hoc Dunnet’s test.