Figure 3.

Transfection with a constitutively active form of Akt prevented the acrolein‐inhibited myogenic differentiation. After the transient transfection of a constitutively active form of Akt (c.a. Akt) or control pcDNA3.1 for 24 h, C2C12 myoblasts were consecutively differentiated in differentiation medium (DM) with 1 μM acrolein (ACR) for 4 days. (A) The protein expressions of Akt, myosin heavy chain (MHC), and myogenin were determined by western blotting and quantified using densitometric analysis. The β‐actin was regarded as a loading control. (B) The activity of creatine kinase is shown. Results are expressed as means ± SEM for at least three independent experiments. ^* P < 0.05 compared with pcDNA control group without acrolein treatment. ^# P < 0.05 compared with pcDNA control group with acrolein treatment.