Fig. 5. Mitochondria from FRDA fibroblasts have higher levels of ROS compared to healthy fibroblasts. Cells were treated (or not) with cpd 2, then with a UVA dose of 250 kJ m^–2 and analysed using the mitochondrial probe mitoSOX red as described in materials and methods. Data were compiled from n = 3–4 measurements per cell line. Mitochondrial ROS levels are indicated as fold change relating to their corresponding unirradiated control cells (taken as 1). * significantly different (p < 0.05) from corresponding unirradiated control cells. † significantly different (p < 0.05) from corresponding sample irradiated with UVA alone.