Figure 6.

Effect of Solamargine in a xenograft mouse model and Solamargine sensitivity (IC₅₀) in primary GC cells from patients. (A) Mice were sacrificed and the tumors were collected. Tumor growth was significantly inhibited following treatment with Solamargine. ^*P<0.05 vs. control. (B) Early apoptosis cells were detected by TUNEL staining. Magnification, ×63. ^*P<0.05 vs. control. (C) H&E staining was used to analyze the necrotic area (%) of the GC xenograft. Upper panel, magnification, ×5; bottom panel, magnification, ×20. ^*P<0.05 vs. control. (D) Primary cells from four patients who underwent radical gastrectomy were treated with appropriate doses of Solamargine for 72 h. (E) Solamargine sensitivity (IC₅₀) in primary GC cells from four patients. GC, gastric cancer; IC50, half-maximal inhibitory concentration (48 h); TUNEL, terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling.