Table II.
Primers used for reverse transcription-quantitative polymerase chain reaction.
| Gene ID | Gene | Primers | Sequence (5′-3′) |
|---|---|---|---|
| 2597 | GAPDH | F | GGAGCGAGATCCCTCCAAAAT |
| R | GGCTGTTGTCATACTTCTCATGG | ||
| 1950 | EGF | F | TCCTCACCCGATAATGGTGGA |
| R | CCAGGAAAGCAATCACATTCCC | ||
| 7039 | TNFA | F | AGATAGACAGCAGCCAACCCTGA |
| R | CTAGGGCCATTCTGCCCATC | ||
| 1839 | HBEGF | F | ATCGTGGGGCTTCTCATGTTT |
| R | TTAGTCATGCCCAACTTCACTTT | ||
| 685 | BTC | F | CTAGGTGCCCCAAGCAATACA |
| R | GCAGACACCGATGACCAAAATA | ||
| 374 | AREG | F | GTGGTGCTGTCGCTCTTGATA |
| R | CCCCAGAAAATGGTTCACGCT | ||
| 2069 | EREG | F | GGACAGTGCATCTATCTGGTGG |
| R | TTGGTGGACGGTTAAAAAGAAGT | ||
| 255324 | EPGN | F | ATGGCTTTGGGAGTTCCAATATC |
| R | TCCTTCTATGTTGTCAGCTTGC | ||
| 7422 | VEGF-A | F | AGGGCAGAATCATCACGAAGT |
| R | AGGGTCTCGATTGGATGGCA | ||
| 7424 | VEGF-C | F | GAGGAGCAGTTACGGTCTGTG |
| R | TCCTTTCCTTAGCTGACACTTGT | ||
| 2919 | CXCL1 | F | AACCGAAGTATAGCCACAC |
| R | GTTGGATTTGTCACTGTTCAGC | ||
| 6374 | CXCL5 | F | AGCTGCGTTGCGTTTGTTTAC |
| R | TGGCGAACACTTGCAGATTAC | ||
| 3576 | CXCL8 | F | ACTGAGAGTGATTGAGAGTGGAC |
| R | AACCCTCTGCACCCAGTTTTC |
F, forward; R, reverse; EGF, epidermal growth factor; HBEGF, heparin-binding EGF-like growth factor; AREG, amphiregulin; EREG, epiregulin; BTC, betacellulin; TNFA, tumor necrosis factor α; EPGN, epithelial mitogen; VEGF, vascular endothelial growth factor.