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. 2019 Mar 19;54(5):1567–1578. doi: 10.3892/ijo.2019.4756

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Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Identification of exosomes. (A) Exosomes were isolated from MSCs and observed using transmission electron microscopy. (B) The western blotting revealed that the CD63 and CD9 proteins were expressed in the exosomes. (C) Confocal microscopy was used to identify the uptake of PKH26-labeled exosomes, which were secreted by N-MSC and internalized in the cytoplasm. N-exo, exosomes secreted by MSCs derived from normal oral mucosa; LK-exo, exosomes secreted by MSCs derived from oral leukoplakia with dysplasia; Ca-exo, exosomes secreted by MSCs derived from oral carcinoma; MSCs, mesenchymal stem cells; CD63, cluster of differentiation 63.