Fig 1. The intracellular proliferation defect of the C. neoformans mutant Δplb1, can be reversed with the addition of exogenous prostaglandin E₂.

J774 murine macrophages were infected with Δplb1, the parental strain H99 or a PLB1 reconstituted ‘rescue’ strain Δplb1:PLB1 (H99 genetic background). Infected cells were left untreated or treated with 2 nM PGE₂ or an equivalent solvent (ethanol) control. Mean IPR from 5 biological repeats shown with error bars representing standard deviation. An unpaired two tailed Student’s t-test was performed to compare each treatment group. H99 etoh vs H99 2 nM PGE₂ ns p = 0.7212, Δplb1 etoh vs. Δplb1 2 nM PGE₂ * p = 0.0376, Δplb1:PLB1 etoh vs. Δplb1:PLB1 2 nM PGE₂ ns p = 0.723.