Fig 4. Comparison of three crypt model implementations and agent descriptions.

(A) Cells are defined on a continuous 2D lattice such that a cell moving off the right edge of the grid reappears on the left edge. Divide and Die gradients are used to describe the behavior of different cell states in the crypt while movement up the crypt is the result of apoptosis. (B) A centroid model may be employed to investigate the role of crypt geometry on the location of anoikis within a crypt. The proliferative state of a cell is defined by prespecified regions along the crypt, and cell death is solely dependent on the occurrence of anoikis. (C) Centroid model that includes differentiation and dedifferentiation between the four main phenotypes present in an intestinal crypt. Wnt signaling is defined by the position within the crypt, whereas Notch signaling is determined by the phenotype of the cell and its neighbors. 2D, two-dimensional.