Table 4. Vaccine-induced peptide specific CD4+ T cell cytokine responses across the period of vaccination.
| Cytokine | Antigen and Group | |||
| ENV | P value* | |||
|
Placebo (n = 4) median (IQR) |
Group A (n = 6) median (IQR) |
Group B (n = 6) median (IQR) |
||
| IFN-γ+ TNF-α+ IL-2+ (Poly functional) |
0.0004 | 0.0016 | 0.0000 | 0.6601 |
| (0–0.021) | (0–0.03) | (0–0.149) | ||
| IFN-γ+ TNF-α+ (Bi-functional) |
0.0000 | 0.0004 | 0.0000 | 0.8640 |
| (0–0.011) | (0–0.09) | (0–0.02) | ||
| IFN-γ+ IL-2+ (Bi-functional) |
0.0153 | 0.0110 | 0.0266 | 0.3334 |
| (0–0.0483) | (0–0.79) | (0–0.24) | ||
| IFN-γ+ (Mono functional) |
0.0156 | 0.0224 | 0.1007 | 0.0686 |
| (0–0.0642) | (0–0.926) | (0–0.833) | ||
| IL-2+ (Mono functional) |
0.0028 | 0.0696 | 0.1570 | 0.1703 |
| (0–0.0916) | (0–0.84) | (0–0.314) | ||
| IL-2+ TNF-α+ (Bi-functional) |
0.0026 | 0.0073 | 0.0000 | 0.0021 |
| (0–0.0314) | (0–0.0159) | (0–0.04) | ||
| TNF-α+ (Mono functional) |
0.0000 | 0.2150 | 0.0184 | 0.0246 |
| (0–0.04) | (0–0.613) | (0–0.55) | ||
| Cytokine | GAG | P value* | ||
| IFN-γ+ TNF-α+ IL-2+ (Poly functional) |
0.0000 | 0.0000 | 0.0000 | 0.7774 |
| (0–0.0127) | (0–0.03) | (0–0.252) | ||
| IFN-γ+ TNF-α+ (Bi-functional) |
0.0000 | 0.0011 | 0.0000 | 0.2051 |
| (0–0.0038) | (0–0.0175) | (0–0.02) | ||
| IFN-γ+ IL-2+ (Bi-functional) |
0.0029 | 0.0113 | 0.0234 | 0.3261 |
| (0–0.0245) | (0–0.0473) | (0–0.245) | ||
| IFN-γ+ (Mono functional) |
0.0053 | 0.0237 | 0.2625 | 0.0032 |
| (0–0.0585) | (0–0.0986) | (0–0.866) | ||
| IL-2+ (Mono functional) |
0.0000 | 0.0783 | 0.2070 | 0.0222 |
| (0–0.404) | (0–0.137) | (0–0.81) | ||
| IL-2+ TNF-α+ (Bi-functional) |
0.0030 | 0.0140 | 0.0000 | <0.001 |
| (0–0.0414) | (0–0.0351) | (0–0.0259) | ||
| TNF-α+ (Mono functional) |
0.0000 | 0.2525 | 0.0095 | 0.0225 |
| (0–0.313) | (0–0.58) | (0–0.252) | ||
| Cytokine | POL | P value* | ||
| IFN-γ+ TNF-α+ IL-2+ (Poly functional) |
0.0000 | 0.0005 | 0.0000 | 0.7609 |
| (0–0.01) | (0–0.0164) | (0–0.0978) | ||
| IFN-γ+ TNF-α+ (Bi-functional) |
0.0006 | 0.0005 | 0.0000 | 0.9454 |
| (0–0.012) | (0–0.00902) | (0–0.0269) | ||
| IFN-γ+ IL-2+ (Bi-functional) |
0.0075 | 0.0081 | 0.0272 | 0.3646 |
| (0–0.0266) | (0–0.04) | (0–0.115) | ||
| IFN-γ+ (Mono functional) |
0.0110 | 0.0210 | 0.0129 | 0.8978 |
| (0–0.05) | (0–0.723) | (0–0.64) | ||
| IL-2+ (Mono functional) |
0.0000 | 0.0729 | 0.0735 | 0.7157 |
| (0–0.0727) | (0–0.648) | (0–0.734) | ||
| IL-2+ TNF-α+ (Bi-functional) |
0.0008 | 0.0047 | 0.0000 | <0.001 |
| (0–0.016) | (0–0.0237) | (0–0.01) | ||
| TNF-α+ (Mono functional) |
0.0025 | 0.1525 | 0.0000 | 0.0114 |
| (0–0.02) | (0–0.618) | (0–0.206) | ||
(*Mann-Whitney Test was used to compare group A and group B at 5% level of significance)