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. 2019 Mar 18;15(3):e1007684. doi: 10.1371/journal.ppat.1007684

Fig 3. Silencing of TRAM, but not MyD88, inhibits phagocytosis of E. coli in human macrophages.

Fig 3

Human primary macrophages (Mϕ) were treated with NS RNA, TRAM siRNA or MyD88 siRNA and stimulated with E. coli or S. aureus bioparticles for 15 min or 15+15 min. Phagocytosis was monitored by 3-D confocal microscopy and presented as mean bacterial count per cell (A) E. coli phagocytosis in Mϕ stimulated for 15 min or 15+15 min. (B) S. aureus phagocytosis in Mϕ stimulated for 15 min or 15+15 min. (C) Phagosome maturation of E. coli- and S. aureus phagosomes in the Mϕ stimulated for 15+15 from Fig 3A and 3B. (D) Phagocytosis of live E. coli and S. aureus in TRAM siRNA treated THP-1 cells. (E) Phagocytosis of live E. coli or S. aureus in MyD88 siRNA treated THP-1 cells. n = number of cells monitored. One-way ANOVA Kruskal-Wallis test (A-C) or Holm-Sidak´s test (D-E) with adj. P values, ** (p < 0.0027), *** (p = 0.0006), **** (p < 0.0001). Red bars: mean ± SD. Data are representative of three independent experiments.