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. 2019 Mar 18;15(3):e1007684. doi: 10.1371/journal.ppat.1007684

Fig 6. FIP2 controls E. coli stimulated activation of Rac1 and Cdc42.

Fig 6

(A) Immunoblot of E. coli stimulated activation of Cdc42 and Rac1 in THP-1 cells treated with NS RNA and FIP2 siRNA. The activation of Cdc42 and Rac1 was monitored by co-incubating glutathione-agarose beads conjugated with GST-PAK1-PBD with lysates of THP-1 cells stimulated with E. coli bioparticles as indicated. (B) Rac1 and Cdc42 levels in lysates from THP-1 cells treated with NS RNA, TRAM siRNA and FIP2 siRNA. (C) Rac1, Cdc42 and FIP2 levels relative to β-tubulin protein levels in wild type THP-1 cells transduced with pLVX-empty- or pLVX-FIP2 vector. (D) Immunoblot of primary human macrophages (M) stimulated with E. coli bioparticles. TRAM antibody conjugated Dynabeads were used for co-precipitation of FIP2 and Rac1 from lysates.