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. Author manuscript; available in PMC: 2019 Dec 1.
Published in final edited form as: Neuropharmacology. 2018 Sep 21;143:113–121. doi: 10.1016/j.neuropharm.2018.09.031

Figure 2. Effect of postsynaptic depolarization on optically-evoked VgatCeA➜ovBNSTGABAA-IPSCs.

Figure 2.

A, Illustration demonstrating ChR2 bi-lateral injections into the CeA of Vgat-Cre mice. B, Illustration demonstrating optically stimulated recordings of ovBNST neurons. C-E, Postsynaptic depolarization in male Vgat-Cre mice injected with ChR2 in the CeA and recorded from ovBNST brain slices in (C) aCSF (n=13 cells/6 mice), (D) in the presence of the non-selective NTR antagonist SR142948 (10μM, n=12 cells/5 mice), (E) SR142948 + the KOR antagonist norNBI (100nM, n=8 cells/2 mice). Insets in C-E are representative optically-evoked GABAA-IPSCs before and after postsynaptic depolarization (double arrows). F, Bar graphs summarizing the proportion of responding neurons to postsynaptic depolarization across different pharmacological treatments. Blue LTP, grey no change and orange LTD. Asterisks, p<0.05.