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. 2019 Mar 28;14(3):e0214680. doi: 10.1371/journal.pone.0214680

Table 1. Primers used for real-time PCR.

Gene Forward primer (5`-3`) Reverse primer (5`-3`)
Nrf2 CTGAACTCCTGGACGGGACTA CGGTGGGTCTCCGTAAATGG
HO-1 GATAGAGCGCAACAAGCAGAA CAGTGAGGCCCATACCAGAAG
TNF-α CCCAGACCCTCACACTCAGATC GCCACTCCAGCTGCTCCTC
IL1β GAGGATACCACTCCCAACAGACC AAGTGCATCATCGTTGTTCATACA
IL-6 CTGCAAGAGACTTCCATCCAGTT AGGGAAGGCCGTGGTTGT
FXR CCAACCTGGGTTTCTACCC CACACAGCTCATCCCCTTT
PPARα GGAGTGCAGCCTCAGCCAAGTT AGGCCACAGAGCGCTAAGCTGT
ACOX1 GCCTTTGTTGTCCCTATCCGT CTTCAGGTAGCCATTATCCATCTCT
MCAD TAATCGGTGAAGGAGCAGGTTT GGCATACTTCGTGGCTTCGT
CPT1 AAGAACATCGTGAGTGGCGTC AGCACCTTCAGCGAGTAGCG
PPARγ ACGCGGGCTGAGAAGTCACG AGTTGGTGGGCCAGAATGGCA
SREBP-1c GGCACTAAGTGCCCTCAACCT TGCGCAGGAGATGCTATCTCCA
FAS CCTGGATAGCATTCCGAACCT AGCACATCTCGAAGGCTACACA
SCD1 GCTGAATAAATCTGCTGTCTTG CCGTGCCTTGTAAGTTCTGTG
β-actin TTCGTTGCCGGTCCACACCC GCTTTGCACATGCCGGAGCC

Nrf2, nuclear factor E2-related factor 2; HO-1, heme oxygenase 1; FXR, farnesoid X receptor; PPARα, peroxisome proliferator-activated receptor α; ACOX1, acyl CoA oxidase 1; MCAD, medium-chain specific acyl-CoA dehydrogenase; CPT1, carnitine palmitoyltransferase I; PPARγ, peroxisome proliferator-activated receptor γ; SREBP-1c, the sterol regulatory element binding protein-1C; FAS, fatty acid synthase; SCD1, stearoyl-CoA desaturase 1.