Table 1. Primers used for real-time PCR.
Gene | Forward primer (5`-3`) | Reverse primer (5`-3`) |
---|---|---|
Nrf2 | CTGAACTCCTGGACGGGACTA | CGGTGGGTCTCCGTAAATGG |
HO-1 | GATAGAGCGCAACAAGCAGAA | CAGTGAGGCCCATACCAGAAG |
TNF-α | CCCAGACCCTCACACTCAGATC | GCCACTCCAGCTGCTCCTC |
IL1β | GAGGATACCACTCCCAACAGACC | AAGTGCATCATCGTTGTTCATACA |
IL-6 | CTGCAAGAGACTTCCATCCAGTT | AGGGAAGGCCGTGGTTGT |
FXR | CCAACCTGGGTTTCTACCC | CACACAGCTCATCCCCTTT |
PPARα | GGAGTGCAGCCTCAGCCAAGTT | AGGCCACAGAGCGCTAAGCTGT |
ACOX1 | GCCTTTGTTGTCCCTATCCGT | CTTCAGGTAGCCATTATCCATCTCT |
MCAD | TAATCGGTGAAGGAGCAGGTTT | GGCATACTTCGTGGCTTCGT |
CPT1 | AAGAACATCGTGAGTGGCGTC | AGCACCTTCAGCGAGTAGCG |
PPARγ | ACGCGGGCTGAGAAGTCACG | AGTTGGTGGGCCAGAATGGCA |
SREBP-1c | GGCACTAAGTGCCCTCAACCT | TGCGCAGGAGATGCTATCTCCA |
FAS | CCTGGATAGCATTCCGAACCT | AGCACATCTCGAAGGCTACACA |
SCD1 | GCTGAATAAATCTGCTGTCTTG | CCGTGCCTTGTAAGTTCTGTG |
β-actin | TTCGTTGCCGGTCCACACCC | GCTTTGCACATGCCGGAGCC |
Nrf2, nuclear factor E2-related factor 2; HO-1, heme oxygenase 1; FXR, farnesoid X receptor; PPARα, peroxisome proliferator-activated receptor α; ACOX1, acyl CoA oxidase 1; MCAD, medium-chain specific acyl-CoA dehydrogenase; CPT1, carnitine palmitoyltransferase I; PPARγ, peroxisome proliferator-activated receptor γ; SREBP-1c, the sterol regulatory element binding protein-1C; FAS, fatty acid synthase; SCD1, stearoyl-CoA desaturase 1.