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. 2019 Mar 6;149(1):41–53. doi: 10.1111/jnc.14646

Figure 4.

Figure 4

Co‐expression of the δ subunit leads to slow muscimol kinetics, particularly very slow dissociation. Association (a) and dissociation (b–d) of [3H]muscimol binding of recombinant α1β2γ2, α6β2γ2 and α6β2δ receptors expressed in HEK293 cells (mean ± SEM; n = 3–6 independent transfections and independent experiments performed in triplicate technical replicates). HEK293 cell membranes were incubated with 5 nM [3H]muscimol at 22°C (a–c) or on ice (d) in the absence or presence of 100 μM GABA determining the non‐specific binding. Dissociation experiments were performed as described in Materials and Methods. The incubations were terminated at various time points by filtration onto GF/B filters. The values are expressed as % of binding at 15 min (a) or 0 min (b–d).