Table 1.

Saturation analysis of [³H]muscimol binding to forebrain and cerebellar membranes of WT and δKO mice at 0°C

	Apparent B max (pmol/mg protein)	Apparent pKD
Forebrain membranes
WT mice	0.66 ± 0.06	8.02 ± 0.06
δKO mice	0.41 ± 0.03**	7.68 ± 0.05**
Cerebellar membranes
WT mice	2.2 ± 0.1	8.34 ± 0.01
δKO mice	1.8 ± 0.1*	8.15 ± 0.02**

Binding of various hot [³H]muscimol concentrations (0.1–30 nM) was measured in triplicate technical samples (3 for total and 3 for non‐specific binding) of WT and δKO mouse membranes at each concentration (mean ± SEM, n = 4 independent experiments, using individual mouse forebrains, and n = 4 independent experiments using samples each pooled of 3 individual cerebella from the mouse line). *p < 0.05; **p < 0.01, significantly different from the corresponding WT value, unpaired t‐test.